Effects of Sesame Oil on the Reproductive Parameters of Diabetes Mellitus-Induced Male Rats

PURPOSE: The purpose of the present study was to investigate the effect of sesame oil on the reproductive parameters of diabetic male Wistar rats. MATERIALS AND METHODS: The adult male rats in a split plot design were divided into normal (n=10), normal 5% (n=5; 5% sesame oil enriched diet), diabetic (Streptozocin induced diabetes; n=9), diabetic 5% (n=9; 5% sesame oil enriched diet), and diabetic 10% (n=9; 10% sesame oil enriched diet) groups. Diet supplementation continued for 56 days. RESULTS: Sesame oil supplementation did not reduce the plasma glucose concentration of rats in the diabetic groups (p>0.05). The total spermatogonia, spermatocytes, Leydig cells/tubule, and the germ cell to Sertoli cell ratio were lower in the diabetic rats than the normal ones (p<0.05), and with the exception of spermatogonia counts, these values improved by the addition of sesame oil to the diet (p<0.05). The sperm progressive motility and viability were lower in the diabetic rats (p<0.05) and sesame oil supplementation did not improve them. Incorporation of sesame oil into the diet improved the plasma testosterone concentration of the diabetic rats in a dose-dependent manner (p<0.05). CONCLUSIONS: In summary, sesame oil supplementation improved the reproductive parameters of diabetic rats at the levels of the testicular microstructure and function, but was not effective in protecting the epididymal sperm.

effects of ex vivo cold-storage on cryopreservation of the goat [Caprus hircus] epididymal sperm

There are many studies focused on long or short storage time of epididymal sperm [EPS] of different species. There are limited studies on preservation or cryopreservation of the domestic goat EPS. The aim of the present study was to evaluate the effects of ex vivo cold-storage on freezing of EPS from goat [Capra hircus]. In a split-plot design the caprine testes-epididymides [40 pairs] were divided to 4 storage-time groups equally [0, 24, 48 and 72 h], then subjected to cryopreservation using Bioxell. Sperm parameters were analyzed before and after freezing. Duration of cold-storage as well as freezing at all storage-time points reduced sperm viability and progressive motility while increased sperm tail abnormalities [p<0.0001]. Freezing reduced the percentage of cytoplasmic droplets [p<0.0001]. The percentage of detached heads was increased at all storage-time points following freezing [p=0.0019], except at time 0 h. It can be concluded that cold storage [in refrigerator [4°C] for 72 h] of epididymides efficiently protected the goat EPS in terms of progressive motility and viability. However, cold-storage may not protect the goat EPS against cryopreservation with Bioxell

Effects of tyrode's solution osmolarities and milk on bull sperm storage above zero temperatures

Control of the medium osmolarity and temperature during long or short time sperm manipulation is essential. The objectives of the present study were to find the effects of different osmolarities of modified Tyrode's solution and milk on the bull sperm during incubation at above zero temperatures. Semen samples were collected twice from five Najdi bull. Centrifuged and most parts of seminal plasma were removed. First experiment: The concentrated semen were splited into nine aliquots to incubate in three different osmolarities [200, 300 and 400 mOsm] at three temperatures [5, 25 and 39°C] for 15 and 60 min of incubation. Second experiment: The semen samples were splited, mixed with the same volume of whole cow milk [5 and 25°C] and milk with 7% glycerol [5°C] and incubated for 15 and 60 min. Sperm motility severely affected [p<0.05] by incubation at low ionic tension [200 mOsm/l] especially at low temperature [5°C]. The impact of low osmolarity on sperm viability can reduce by increasing the incubation temperature to 39°C. The decreased sperm motility, which was induced by lowering osmolarity, was not improved [p>0.05] by increasing temperature during 1 h of incubation. Milk can protect the sperm viability and motility at cool conditions and there is no beneficial effect of glycerol in combination of milk on sperm incubation at above zero temperatures [p<0.05]. Iso- and hyper-osmotic solutions protect bull sperm motility and viability at 25 and 39°C, while milk can be used for protecting sperm at 5°C